麦草热水预水解过程产物分析及木糖生成模型

Analysis of the Products Wheat Straw Autohydrolysis and the Model of Xylose Generation

关键词:  热水预水解  麦草秸秆  木糖  响应面  发酵抑制物

Key Words:autohydrolysis  wheat straw  xylose  response surface  fermentation inhibitor

摘要:为了探究麦草热水预水解过程中产物的生成变化规律，分别采用离子色谱和高效液相色谱检测预水解液中糖类（阿拉伯糖、半乳糖、葡萄糖和木糖）和发酵抑制物（甲酸、乙酸、5-羟甲基糠醛和糠醛）的含量，分析了单因素实验中糖类和发酵抑制物的生成变化规律；同时采用响应面法对影响麦草热水预水解液中木糖生成的固液比、水解温度和水解时间进行了优化研究，建立了木糖生成量的二次多项式数学模型。结果表明，在固液比1∶11.67、水解时间57.76 min和水解温度173.96℃的条件下，木糖的生成量达到最高，为83.91 mg/g麦草。

Abstract:In order to explore the product change in the process of wheat strawautohydrolysis, Ion chromatography and high performance liquid chromatography (HPLC) were used to detect sugars(arabinose, galactose, glucose and xylose) and fermentation inhibitors (formic acid, acetic acid, 5-HMF and furfural) in the hydrolysate, the change rules of sugars and fermentation inhibitors in the single factor experiment were analyzed. Liquid ratio，temperature and time parameters which impact on xylose generation in the auto-hydrolysate were researched through the response surfaceanalysis.And mathematical model of quadratic polynomial of xylose generation was established. The results showed that under the optimum conditions (liquid ratio 1∶11.67, time 57.76 min and temperature 173.93℃), the xyloseextraction efficiencyof wheatgrass was the highest, reached to 83.91 mg/g.