内切纤维素酶预处理对漂白马尾松纤维形态和结构的影响

Study on Fiber Modification of Bleached Masson Pine by Endo-cellulase and Its Mechanism

关键词:  内切纤维素酶  纤维素微纤丝  纤维形态  结晶度  纤维性能

Key Words:endo-cellulase  cellulose microfibril  fiber morphology  crystallinity  fiber properties

摘要:针对制备纤维素微纤丝(CMF)所需要高能耗的问题,采用内切纤维素酶对漂白马尾松纤维进行预处理,研究内切纤维素酶预处理过程中,纤维形态、纤维素聚集态和纤维性能所发生的变化。结果表明,内切纤维素酶优先作用于纤维表面，不会对纤维产生明显的起皮、剥皮现象,但是对纤维的切断作用较强，导致内切纤维素酶用量增大时,纤维的平均长度逐渐减小,宽度增加,保水值和Zeta电位逐渐下降,打浆度在酶预处理初期略有下降,之后不断增大;内切纤维素酶主要作用于纤维素的无定形区,使纤维素的结晶度增大,最后在80%~82%之间波动；若要纤维素完全发生水解，还需外切纤维素酶和纤维二糖酶的协同作用。

Abstract:The effect of endo-cellulase hydrolysis on fiber morphology,cellulose aggregation structure and fiber properties of bleached masson pine was investigated. The results showed that endo-cellulase preferentially reacted with the fines on fiber surface. Peeling was not very obvious, but cutting effect was significant. With the increasing of dosage, average fiber length, water retention value and absolute value of Zeta potential decreased gradually and the width changed by contraries. The beating degree decreased at first and then gradually increased. Hydrolysis mainly occurred at the amorphous region, so the crystallinity increased and finally fluctuated between 80%～82%. The fiber could be hydrolysed completely only under the synergistic effect of endo-cellulase, exo-cellulase and cellobiase.