降解天然树脂的微生物分离与筛选

Separating and Screening of Microorganism for Degrading Natural Resin

关键词:  树脂障碍  天然树脂降解  微生物  分离与筛选

Key Words:pitch troubles  natural resin degrade  microorganism  separationg and screening

摘要:从造纸厂采集的土样中，经过富集、初筛和多轮复筛实验，分离得到了5株降解天然树脂能力较强的微生物。采用液体发酵以及稳定性复筛的方法，从这5株微生物中筛选得到了降解天然树脂能力稳定并且降解率较高的菌株Z75、Z37。分别对这2株菌株进行形态学观察,培养24 h后的菌落直径均大于1 mm，菌株Z75菌体长度小于5 μm，初步判断Z75菌株为短杆菌；菌株Z37菌体长度小于0.1 μm，初步判断Z37为细球菌。对Z75菌株的降解条件进行研究，结果显示,在35 mL的Z75菌株发酵液中，最适降解反应的树脂底物颗粒（球形）质量为0.2 g，最适降解反应时间≥20 h；发酵条件和培养基初步优化的结果显示，Z75菌株发酵液降解天然树脂的降解率由优化前的6.5%左右提高到43.3%。

Abstract:After enrichment, primary screening and stable repeat screening five strains of microorganism from the soil sample provided by Nanping Paper Co., Ltd were separated, which can degrade natural resin. Using the method of liquid fermentation and stable repeat screening two strains with high degradation ability—Z75, Z37 were screened from the five strain microorganisms. After 24 h cultivation, the Morphology study of the two strains showed that the diameter of the bacterial agglutinin is >1mm,the length of the thalli is <5 μm. It is preliminary estimated that the Z75 is brevibacterium.sp and the Z37 is micrococcus varians.The primary study of degradation conditions for Z75 indicated that the optimum particle of natural resin is 0.2 g while the most appropriate cultivation time is 20 h or more. As a result of optimizing fermentation conditions and primary adjustment of culture medium, the degradation rate of Z75 rise from 6.55% to 43.3%, which is significant for using to degrade the natural resin.