亚硫酸铵预处理提高麦草备料废渣的酶水解效率

Enhancement of the Enzymatic Saccharification of the Residues from Wheat Straw Preparation by Ammonium Sulfite Pretreatment

关键词:  麦草备料废渣  亚硫酸铵  预处理  化学成分  酶水解糖化

Key Words:wheat straw handling residue  ammonium sulfite  pretreatment  chemical composition  enzymatic saccharification

摘要:为高效经济利用制浆厂的备料废渣,研究了亚硫酸铵预处理对麦草备料废渣主要化学成分及酶水解糖化效率的影响。结果表明,原料的木素脱除率随预处理温度升高和亚硫酸铵用量增加而提高,半纤维素的降解受温度影响较大,纤维素在预处理中几乎没有降解。在本研究范围内,随着预处理强度的增大,碳水化合物的酶水解糖转化率先上升后下降,相比于提高温度,增加亚硫酸铵用量对提高酶水解效率的影响更为显著。麦草备料废渣在165℃下用16%亚硫酸铵预处理,经PFI磨磨浆得到的底物,其酶水解(以聚葡萄糖为基准的酶用量为40 FPU/g)聚葡萄糖和聚木糖转化率分别为88.9%和44.9%,总糖转化率为67.0%。

Abstract:The effects of ammonium sulfite pretreatment on chemical composition and enzymatic saccharification of the residue from wheat straw preparation were investigated. The results revealed that more lignin was removed with the temperature and the charge of ammonium sulfite increasing. The degradation of hemicellulose increased with the increasing of the temperature distinctly, while the cellulose remained stable during the process of pretreatment. The increasing of the temperature and the charge of ammonium sulfite was beneficial to enhance the enzymatic saccharification. The sugar conversion of pretreated the residue from wheat straw increased with the increase of ammonium sulfite charge and temperature, the charge of ammonium sulfite was more sensitive than the temperature. The glucan and xylan conversion reached up to 88.9% and 44.9%, respectively, and the highest total sugar conversion was 67.0%, when the pretreated substrate with 16% ammonium sulfite charge, and 165℃ was hydrolyzed at a cellulase dosage of 40 FPU per gram of cellulose in substrate.