草酸预处理麦秆工艺优化及其糖化发酵产氢研究

Optimization of oxalic acid pretreatment of wheat straw for hydrogen production by saccharification and fermentation

关键词:  小麦秸秆  草酸  同步糖化发酵(SSF)  生物制氢  响应面法

Key Words:Wheat straw  Oxalic acid  Simultaneous saccharification and fermentation (SSF)  Biohydrogen  Response surface methodology

摘要:为了实现农业秸秆废弃物的综合利用，本研究采用Box-Behnken设计和基于三变量中心组合设计的响应面法，对草酸溶液预处理小麦秸秆的工艺进行了优化，建立了小麦秸秆葡萄糖转化率与预处理条件之间的数学模型，并验证了该模型的有效性。结果表明，预处理的反应时间和温度是影响小麦秸秆酶解效果的显著因素。葡萄糖转化的最佳预处理条件是草酸与水 的摩尔比为1:3.8，固液比为1：50，反应温度为110℃，保温时间为20 min，该条件下纤维素的葡萄糖转化率可达95.1%，与响应面分析预测的葡萄糖转化率（94.5%）一致。此条件下处理后的秸秆同步糖化发酵产氢量可达131.5 mL/g。该模型对草酸预处理农业废弃物的商业化利用具有指导意义。

Abstract:In order to realize the comprehensive utilization of agricultural waste, in this study, oxalic acid solution pretreatment was investigated and optimized for wheat straw saccharification using Box-Behnken design and response surface methodology based on a three-variable central composite design. A mathematical model between glucose conversion rate of wheat straw and pretreatment conditions was established and the validity of the mathematical model is verified. The results indicated that the reaction time and temperature of the pretreatment were significant factors that influenced the enzymatic hydrolysis effect of wheat straw. The optimum process conditions for glucose conversion are an oxalic acid to water ratio of 1:3.8, solid to liquid ratio of 1:50, reaction temperature of 110 ℃, and heat preservation time of 20 min, the conversion of glucose arrived at 95.1%, which is consistent with the saccharification efficiency predicted (94.5%) by response surface analysis. Under that condition, the yield of biohydrogen by simultaneous saccharification and fermentation can reach 131.5 mL/g. The results indicate that this model has guiding significance for the commercial utilization of agricultural waste by oxalic acid pretreatment.